Figure 1.

The workflow of sampling and isolation. The black biofilms samples from particular areas of bathroom, kitchen, refrigerator rubber seal, and washing machine were taken by using sterile cotton swabs; the black biofilm materials were cultured on PDA medium (200 mg/L chloramphenicol and 200 mg/L cycloheximide) for 2 weeks. Susceptive black yeast like single colony strains were taken for further culture under same condition as above.