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. 2018 May 16;8:7712. doi: 10.1038/s41598-018-26086-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison and overlay of the microCT scan and histological image of the resin embedded and laser cut mouse lung sample. (A) Shows an exemplary slice of the reconstructed PHR microCT data set of the resin embedded PTA stained mouse lung. In addition to alveolar lung tissue, a lung metastasis (m), the oesophagus (o), larger bronchi (b) and blood vessels (v) are displayed. (B) Shows the microscope image of the Sandersons Rapid Stain (SRS) stained and van Gieson (VG) counterstained cut performed with the laser microtome at the predefined position. No cutting artefacts are visible. (C) Depicts the overlay of both data sets. The histological data is shown in pseudo SRS/VG colours (the data set was colourised for better visualisation), while the CT data is shown in green. The overlay of the CT data with the histology image reveals a precise match of both data sets.