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. 2018 May 16;8:7661. doi: 10.1038/s41598-018-25654-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of ODN-mediated genome edits by edit-specific PCR and restriction analysis. (A) Shown are amplification results for the nine bp deletion-specific PCR and a control amplicon for an endogenous gene (α-lactalbumin) with DNA isolated from a blood sample from the two live calves 1601 and 1602. PC: positive plasmid control with nine bp LGB deletion insert; WT: genomic wild type DNA; H₂O: water; M: DNA size marker. (B) Results of a diagnostic restriction enzyme digest with SfoI on blood and ear derived DNA from calves 1601 and 1602 and a wild type DNA sample. The black arrows point to two diagnostic restriction fragments indicative of an unedited wild type sequence. The bottom gel shows the undigested PCR fragments. The gel images in panels A and B were cropped for improved clarity. The respective full size gel photos are shown in Supplementary Figure S3.