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. 2018 May 16;8:7661. doi: 10.1038/s41598-018-25654-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Deep sequencing analysis of the edited LGB locus in the calves 1601, 1602 and 424 and corresponding embryo biopsies. DNA isolated from blood, ear fibroblasts (ear), a selection of tissues (as indicated) and embryo biopsies (biopsy) were analyzed for the presence of TALEN-mediated edits and unaltered wild type sequences of the LGB target locus by enumerating corresponding sequencing reads for 1601, 1602, 424 associated samples in addition two five wild type embryo samples (1, 2, 3, 7, 8). The bars depict the percentage of all joined paired end sequence reads in the indicated samples for the HDR-generated nine bp deletion allele (red), wild type allele (blue) and additional 21 bp (grey), 24 bp (yellow) and 66 bp (black) deletion alleles that were identified by the deep sequencing analysis. The values that were used to generate the graph can be found in Supplementary Table S2. Error bars indicate standard errors.