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. 2018 May 16;8:7661. doi: 10.1038/s41598-018-25654-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amount of BLG in induced milk of the genome edited LGB knockout calf 1601. BLG levels in the milk of calf 1601 were assessed by (A) Coomassie Blue staining and (B) western analysis following SDS-PAGE separation of milk samples. Each lane of the gel was loaded with an equal amount of milk. WT: natural milk from a single wild type cow; WTP: pooled natural milk samples from several wild type cows; 1601 d4 and d5: milk sample from 1601 on the fourth and fifth day of an induced lactation; KD: milk sample from a transgenic line of LGB knockdown cattle. Positions of the main milk proteins and a smaller form of BLG (BLG*) observed by western are indicated. α_s-CN: α_s-casein; β-CN: β-casein; κ-CN: κ-casein; BLG: β-lactoglobulin; α-Lac: α-lactalbumin. Please note, the images shown in panels A and B were cropped for improved clarity. The respective full size photos of the stained gel and western membrane are shown in Supplementary Figure S4.