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. 2018 May 16;8:7664. doi: 10.1038/s41598-018-25949-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lifespan Assays in hKv9.2 Transgenic Lines. (A) Expression of hKv9.2 and hKv9.2-D379E channels throughout neuronal development and effects on lifespan. A total of 200 virgin flies per line were sex-segregated within 4 h of eclosion and maintained in small laboratory vials (n = 20 per vial) containing fresh food in a low-temperature incubator at 25 °C and 40% humidity on a 12/12 h dark/light cycle. The flies were then transferred to fresh food vials every 3-4 days and mortality recorded. Significant differences were not observed between Elav/+ and Elav > hKv9.2 (p = 0.8771) or Elav/+ and Elav > hKv9.2-D379E (p = 0.0656). (B) Expression of hKv9.2 and hKv9.2-D379E channels post developmentally in neurons and effects on lifespan. A total of 100 virgin flies per line were sex-segregated within 4 h of eclosion and age-matched flies were maintained in small laboratory vials (n = 10 per vial) containing fresh food in a high-temperature incubator at 29 °C and 40% humidity on a 12/12 h dark/light cycle. The flies were then transferred to fresh food vials every 2-3 days and mortality recorded. Significant differences were observed between Elav-gal4/+Gal80^ts and Elav-gal4 > Gal80^ts hKv9.2 (p = 0.0012) or Elav-gal4/+Gal80^ts and Elav-gal4 Gal80^ts > hKv9.2-D379E (p = 0.0001).