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. 2018 May 16;8:7717. doi: 10.1038/s41598-018-26098-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Suggested diagnostic workflow. The overview image was taken at amid I (1656 cm⁻¹), showing tissue morphological characteristics. Upper right, a large lymph follicle; center, glandular tumor lesion; yellow rectangle, region of interest. Bottom, single field analyses. Left, H&E staining; middle, first-level RF analysis; red, pathological regions; yellow, the invading inflammatory cells; white, the surrounding muscles; green, connective tissue. Right, second level RF analysis. All regions marked red using the first level RF were further analyzed by the second level RF of the selected, single-field, region: red, tumor cells; magenta, necrotic regions; yellow, infiltrating inflammatory cells.