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. 2018 May 16;8:7670. doi: 10.1038/s41598-018-25998-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pax3^EGFP expression in mouse embryonic forelimbs. (a) Fluorescent microscopy showing Pax3^EGFP expression based on a Pax3^Cre|Rosa26^EGFP driver at E11, E12, E13, and E14 forelimbs. (b) Scatter plots from FACS showing EGFP intensity on the x-axis, and forward scatter (FSC) on the y-axis. Gate R5 shows 92%, 92%, 68%, and 61% EFGP-positive cells in forelimbs at E11, E12, E13, and E14, respectively. (c) Histograms depict EGFP intensity on the x-axis vs cell number (count) on the y-axis. Green peaks represent EFGP-positive populations based on gating from R5.