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. 2018 May 16;8:7670. doi: 10.1038/s41598-018-25998-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myogenic and non-myogenic cells populate the Pax3^EGFP lineage in the forelimb. (a–d) Immunohistochemistry of E11 (a,a1,c,c1) and E12 (b,b1,d,d1) forelimb frontal sections of wild type mice for Pax3, Myf5 and Myog. (a1–d1) higher magnification of the a–d images. (e) Immunohistochemistry of E11 forelimb frontal sections of Pax3^EGFP mice for EGFP (Pax3), Myog and Pitx2. (e1) higher magnification of the e image. (f,g) Immunohistochemistry of E12 forelimb frontal sections of Pitx2^Z/+ (f) and Pitx2^Z/+|Pax3^Sp/Sp (g) mouse for ß-gal(Pitx2) and Pax3.