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. Author manuscript; available in PMC: 2019 May 23.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Apr 9;499(4):751–757. doi: 10.1016/j.bbrc.2018.03.209

Fig.2. nSMase2 inhibitors suppress release of EVs from P2-seeded tau biosensor cells.

Fig.2

Four groups of samples were analyzed: Lipo/DMSO samples contained EVs released from non-seeded (empty liposome treated) cells after 48 hours of vehicle (DMSO) treatment, P2/DMSO, P2/Camb, and P2/GW4869 - EVs released from P2-seeded tau biosensor cells after 48 hours of treatment wth DMSO, cambinol (50μM) or GW4869 (50μM) treatment respectively. EV samples purified from the same number of donor cells were analyzed per each group. (A) Purified EVs were imaged by transmission electron microscopy. (B) Size distribution of particles in the samples were assessed by nanoparticle tracking analysis. (C) WB analysis for exosome markers, representative images. (D) Densitometry analysis from WB. Histograms represent average relative signal intensity (mean ± SEM) from three independent experiments. * p<0.05, ** p<0.01.