Skip to main content
. 2018 May 16;3(3):e00061-18. doi: 10.1128/mSphere.00061-18

TABLE 2 .

Primary articles describing studies that utilized Lactobacillus to express bacterial antigensa

Pathogen Lactobacillus
species		 Antigen(s)
expressed		 Expression Result(s) Intended
host(s)		 Reference
Bacillus anthracis L. gasseri PA Unknown Serum IgG, mucosal IgA, T cell response Human,
animal		 71
Bacillus anthracis L. gasseri PA Unknown Neutr. Ab, T cell response, challenge Human 70
Bacillus anthracis L. acidophilus PA Surface Neutr. Ab, mucosal IgA, challenge Human 69
Bacillus anthracis L. casei PA Surface,
intracell.,
secreted		 Serum IgG Human 68
Bacillus anthracis L. acidophilus PA Surface Stability Human 114
Borrelia burgdorferi L. plantarum OspA Surface Serum IgG, mucosal IgA Human 83
Borrelia burgdorferi L. plantarum OspA Unknown Serum IgG, mucosal IgA, challenge Human 82
Bordetella pertussis L. casei FHA Intracell. Serum IgG Human 115
Clostridium botulinum L. acidophilus BoNT/A-Hc Surface Stability Human 114
Clostridium perfringens L. casei ε-Toxoid Surface Serum IgG, serum IgA, intestinal IgA, IFN-γ,
challenge		 Human,
animal		 86
Clostridium perfringens L. casei α-, β1-, β2-,
ε-toxoids		 Unknown Serum IgG, fecal IgA, nasal IgA, IFN-γ/IL-4,
T cell response, challenge		 Human,
animal		 116
Clostridium perfringens L. casei β-Toxoid Surface,
intracell.		 Serum IgG, serum IgA, intestinal IgA, IFN-γ,
challenge		 Human,
animal		 117
Clostridium perfringens L. casei α-Toxoid Surface Serum IgG, mucosal IgA, challenge Human,
animal		 118
Chlamydia psittaci L. fermentum OmpA Surface Stability Animal 97
Clostridium tetani L. casei TTFC Surface,
intracell.,
secreted		 Serum IgG Human 119
Clostridium tetani L. plantarum TTFC Intracell. Serum IgG, mucosal IgA Human 120
Clostridium tetani L. plantarum TTFC Intracell. Serum IgG Human 121
Clostridium tetani L. johnsonii TTFC Surface Serum IgG, mucosal IgA Human 122
Clostridium tetani L. plantarum TTFC Intracell.,
secreted,
surface		 Serum IgG, mucosal IgA Human 123
Clostridium tetani L. plantarum TTFC Intracell. Serum IgG, mucosal IgA, T cell response,
challenge		 Human 124
Clostridium tetani L. plantarum,
L. casei 		TTFC Intracell.,
surface		 Serum IgG, mucosal IgA, T cell response Human 125
Chlamydia trachomatis L. plantarum,
L. fermentum 		VD4 Surface Stability Human 126
Chlamydia trachomatis L. plantarum Hirep2 Surface Serum IgG, serum IgA, mucosal IgA, IFN-γ Human 90
Escherichia coli (EHEC
O157:H7)		 L. acidophilus EspA,
Tir		 Secreted Serum IgG, mucosal sIgA, IFN-γ, IL-4, IL-10,
challenge		 Human 127
Escherichia coli (EPEC) L. casei β-Intimin Unknown Serum IgG, mucosal IgM, challenge Human 77
Escherichia coli (ETEC) L. casei K88 Unknown Serum IgG, mucosal sIgA, challenge Human 128
Escherichia coli (ETEC) L. casei FaeG Secreted Stability Human 129
Escherichia coli (ETEC) L. casei FP Secreted Stability Human 129
Escherichia coli (ETEC) L. casei F1 Surface Serum IgG, mucosal IgA, challenge Swine,
ruminants,
human		 75
Escherichia coli (ETEC) L. casei K88, K99 Surface Serum IgG, mucosal IgA, T cell response Swine,
ruminants,
human		 74
Escherichia coli (ETEC) L. casei K99 Surface Serum IgG, mucosal IgA Swine,
ruminants,
human		 73
Escherichia coli (ETEC) L. casei F41 Surface Serum IgG, mucosal IgA, T cell response Swine,
ruminants,
human		 72
Escherichia coli (ETEC) L. reuteri ST, LT(B) Secreted Serum IgG, mucosal IgA, challenge
protection		 Swine,
ruminants,
human		 76
Escherichia coli (ETEC) L. acidophilus K99 Surface In vitro inhibition of pathogen adhesion Swine,
ruminants,
human		 130
Escherichia coli (ETEC) L. plantarum Fimbrial
adhesin
(FaeG)		 Unknown Serum IgG, intestinal IgA, challenge Swine,
ruminant,
human		 131
Escherichia coli (UPEC) L. reuteri PapG Surface Stability Human 132
Helicobacter pylori L. acidophilus Hp0410 Unknown Serum IgG, mucosal IgA, challenge Human 85
Helicobacter pylori L. acidophilus Hp0410 Surface Stability Human 133
Helicobacter pylori L. plantarum UreB Unknown Serum IgG, serum IgA, challenge Human 134
Mycobacterium avium
(MAP)		 L. salivarius MMP Surface Stability Ruminant 135
Mycobacterium avium
(MAP)		 L. salivarius ptD Intracell. Stability Ruminant 136
Mycobacterium tuberculosis L. plantarum Ag85B,
ESAT-6		 Surface Mucosal IgA, T cell response Human 137
Salmonella enterica (SE) L. casei FliC, SipC Surface Serum IgG, T cell response Human,
animal		 138
Salmonella enterica (SE) L. casei FliC Surface Challenge Human 139
Streptococcus mutans L. zeae scFv Surface,
secreted		 Challenge Human 140
Streptococcus pneumoniae L. casei PspC Surface,
intracell.		 Mucosal IgA, challenge Human 141
Streptococcus pneumoniae L. casei PspA, PspC Surface Serum IgG, mucosal IgA Human 81
Streptococcus pneumoniae L. casei PspA Surface Serum IgG, challenge Human 80
Streptococcus pneumoniae L. casei,
L. plantarum,
L. helveticus 		PspA Surface Serum IgG, mucosal IgA, challenge Human 79
Streptococcus pneumoniae L. casei PsaA, PspA′1,
PspA′3		 Intracell.,
secreted		 Stability Human 142
Streptococcus pyogenes L. gasseri CRR6 Unknown Serum IgG, mucosal IgA, challenge Human 143
Streptococcus pyogenes L. sake,
L. fermentum 		M6 Secreted,
surface		 Stability Human 144
V. cholerae L. casei,
L. reuteri 		CTB Intracell.,
secreted		 Serum IgG Human 145
Vibrio parahaemolyticus L. rhamnosus MAM-7 Unknown MAM-7 expression (reduced Lactobacillus
ability to inhibit pathogen)		 Human 146
Vibrio parahaemolyticus L. rhamnosus MAM-7 Unknown Stability Human 146
Yersinia pestis L. plantarum LcrV Surface Serum IgG, mucosal IgA, T cell response Human 84
Yersinia pseudotuberculosis L. plantarum D1-D5, D4-D5 Surface Stability Human 147
a

FHA, filamentous hemagglutinin adhesin; BoNT, clostridial botulinum neurotoxin; TTFC, tetanus toxin fragment C; FP, fusion protein; MMP, mucous membrane pemphigoid; intracell., intracellular; Neutr. Ab, neutralizing antibody.