Figure 3.

Suppression of cisplatin-induced FGF21 expression by pifithrin-α. BUMPT cells were treated with cisplatin (20 μM) in the presence or absence of pifithrin-α (20 μM). A) Representative images of cellular and nuclear morphology. Scale bar, 50 μm. B) Percentage of apoptosis. Means ± sd (n = 3). *P < 0.0005 vs. untreated control cells, ^#P < 0.005 vs. cisplatin-only treated KD group cells. C) Representative immunoblots of FGF21, p-P53, P53, C-caspase 3, and β-actin as protein loading control.