Figure 3.

Acer2 knockout reduces SPH, DHS, S1P, and DHS1P levels in whole blood, plasma, serum, erythrocyte, and platelets. A) Whole blood samples were collected into EDTA-coated tubes from Acer2^+/+ or Acer2^−/− male mice at age 2 mo and were subjected to LC-MS/MS analyses for SPH, DHS, S1P, DHS1P, ceramides (Cer), dihydroceramides (dhCer), sphingomyelins, and monohexasylceramides. Data represent means ± sd (n = 3 mice/genotype). B–D) Platelet-less plasma (B), erythrocytes (C), and platelets (D) were prepared from whole blood samples collected as in panel A and were subjected to LC-MS/MS analysis for SPH, dhSPH, S1P, dhS1P, ceramides, and dihydroceramides. Pi, inorganic phosphate. Data represent means ± sd (n = 3 mice/genotype).