Figure 2.

Effect of shifted LD cycles on clock gene oscillations in lymphoid tissues and cells. Representative temporal patterns of ensemble PER2::LUC bioluminescence recorded from individual cultures of spleen (A) adipose (B) tissue SVCs from HFD-fed mPer2^Luc mice that were maintained in fixed (black) or shifted (red) LD 12:12 cycles. Bar graphs depict comparisons of the circadian period (means ± sem) of the PER2::LUC rhythms in spleen (n = 9) and SVC (n = 6) cultures from fixed and shifted LD mice. Asterisks indicate that the period of the PER2::LUC rhythms in cultures from shifted LD mice was significantly decreased (P < 0.05) relative to that observed in lymphoid tissue and cells from fixed LD controls.