Figure 5.

Effect of shifted LD cycles on SVC expression of proinflammatory cytokines in HFD-fed mice. SVC cultures were isolated from HFD-fed mice on fixed or shifted LD cycles and collected (n = 3–4) at 6, 12, 18, and 24 h after serum shock administration for real-time PCR analysis of IL-1β (A), IL-6 (B), and TNF-α (C) mRNA levels. Plotted values correspond to the ratios of IL-1β, IL-6, or TNF-α/β-actin mRNA signal (means ± sem) that were adjusted in relation to the average for the fixed LD group. Asterisks denote time-based comparisons in which the relative levels of IL-1β, IL-6, and TNF-α mRNA in SVC cultures from HFD-fed mice on shifted LD cycles were significantly increased (P < 0.05) compared with those found in fixed LD controls.