Gas chromatogram showing the fatty acid methyl ester profile at different stages of incubation. The γ-linolenic acid (GLA) peak at retention time of 21.8 min on the 3rd, 4th and 5th day of incubation, respectively: a) not observed, meaning that there were no lipids, b) γ(GLA)=882 mg/L, and c) γ(GLA)=866 mg/L