Figure 4.

H3F3A Mutant Subgroups

(A–D) H3F3A_G34R/V. (A) Radiological tumor lesion map of 7 H3F3A_G34R/V cases. Brighter colored pixels indicate a higher probability of tumor incidence. (B) Integrated annotation of somatic mutations and DNA copy-number changes in H3F3A_G34R/V cases. Clinicopathological and molecular annotations are provided as bars according to the included key in Figure 3. (C) H&E (top) and immunohistochemistry (bottom) directed against H3.3G34R for HERBY020. Scale bars, 50 μm. (D) Kaplan-Meier plot of event-free and overall survival of 55 hemispheric cases (y axis) separated by H3F3A status, time given in months (x axis), p value calculated by the log rank test.

(E–H) H3F3A_K27M. (E) Radiological tumor lesion map of 21 H3F3A_K27M cases. Brighter colored pixels indicate a higher probability of tumor incidence. (F) Integrated data from H3F3A_K27M cases. Clinicopathological and molecular annotations are provided as bars according to the included key in Figure 3. (G) H&E (top) and immunohistochemistry (bottom) directed against H3.3K27M for HERBY015. Scale bars 50 μm. (H) Kaplan-Meier plot of event-free and overall survival of 34 midline cases (y axis) separated by H3F3A status, time given in months (x axis), p value calculated by the log rank test.

See also Figure S4.