Fig. 1.

Image capture, vessel detection, and flow sequence estimation. All video image frames are 640 × 480 pixels with a resolution of 1 μm per pixel. Frames are stitched in software to produce mosaic images across the whole nailfold. Panels are as follows: (1) An overview of the microscope system and capture software interface; (2) Diagram of motor positions and tracking data used to inform frame stitching and the mosaic creation process; (3) A fully-registered mosaic image with automatically detected vessels highlighted, counted and measured; (4) Individual regions from the mosaic in (3), showing vessel region detection (left) and vessel path orientation (right); (5) Structural mosaic from (3) overlaid with false colour flow information extracted from vessels using optical flow techniques.