Table 3.

Metabolites with significant differences among feeding, 24 h of fasting and 48 h of fasting^a

No.	Name	m/z	Formula	Fold changeb			Pathway analysis
				FD/FS_24	FD/FS_48	FS_24/FS_48
1	12,13-DHOME	297.2420	C18H34O4	0.30	0.49	1.62	Linoleic acid metabolism
2	Linoleic acid	263.2366	C18H32O2	0.17	0.20	1.18	Linoleic acid metabolism
3	Stearidonic acid	277.2157	C18H28O2	0.11	0.11	1.02	alpha-Linolenic acid metabolism
4	Oleic acid	300.2893	C18H34O2	0.38	0.62	1.62	Fatty acid metabolism
5	Palmitoleic acid	255.2314	C16H30O2	0.21	0.25	1.17	Fatty acid metabolism
6	Pantothenic acid	220.1178	C9H17NO5	1.22	0.72	0.59	Pantothenate and CoA biosynthesis
7	Glycerophosphocholine	280.0917	C8H20NO6P	1.75	1.51	0.86	Ether lipid metabolism
8	LysoPC(O-18:0)	510.3912	C26H56NO6P	1.02	0.34	0.33	Ether lipid metabolism
9	Sphinganine	284.2942	C18H39NO2	0.37	0.46	1.24	Sphingolipid metabolism
10	5-Aminopentanoic acid	118.0864	C5H11NO2	1.30	0.84	0.65	Lysine degradation
11	Aminoadipic acid	144.0654	C6H11NO4	1.32	0.83	0.63	Lysine degradation
12	Betaine	140.0681	C5H11NO2	1.87	1.05	0.56	Glycine, serine and threonine metabolism
13	Ornithine	133.0972	C5H12N2O2	2.00	1.64	0.82	Arginine and proline metabolism
14	L-Glutamine	169.0582	C5H10N2O3	2.12	1.66	0.78	Arginine and proline metabolism
15	L-Tyrosine	199.1076	C9H11NO3	0.49	0.45	0.93	Nitrogen metabolism

^aFD: Feeding; FS_24: 24 h fasting; FS_48: 48 h fasting; 12,13-DHOME: 12,13-dihydroxyoctadecenoic acid; LysoPC(18:0): lysophosphatidylcholine 18:0

^bFold change was calculated by dividing the mean of normalized intensity of each plasma metabolite in the former by the mean of normalized intensity of each plasma in the latter. Fold change > 1 indicates that the metabolite was down-regulated, whereas fold change < 1 indicates the metabolite was up-regulated