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. 2018 May 16;20:41. doi: 10.1186/s13058-018-0966-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Net1 deletion inhibits tumor angiogenesis and causes increased tumor cell death. a Representative examples of H&E staining of large tumors in genotypes shown. b Quantification of necrotic area in H&E sections over range of tumor sizes for genotypes shown. Five animals per genotype, 4–5 tumors per animal, quantified. c Quantification of necrotic area of all tumors per genotype. d Representative examples of cleaved caspase 3 (CC3) staining in large tumors from genotypes shown. e Quantification of number of CC3 hot spots in large tumors. Entire tumor sections analyzed from 8 mice of each genotype. f Examples of cell determinant 31 (CD31) staining (red) in solid tumors from Net1^+/+,PyMT and Net1^−/−,PyMT mice. DNA shown in blue. g Quantification of CD31 staining. Five areas per sample analyzed; tumors from 4 (Net1^+/+,PyMT) or 5 (Net1^−/−,PyMT) mice assessed. Bars represent median values. *P < 0.05; **P < 0.01; ***P < 0.001. H&E hematoxylin and eosin, Net1 neuroepithelial transforming gene 1, PyMT polyoma middle T antigen