Table 1.
Viral clones and stocks used in this study
| Clones | Viral stock namea | Major mutation | Effect on replication (CrFK) |
|---|---|---|---|
| pCF-7 [25] | Wild-type FFVb | – | – |
| pCF7-BBtr | FFV-BBtr | Truncation at Bet amino acid 117 | Fully susceptible towards feA3-mediated restriction in vitro |
| pCF7-BetMCS [50] | FFV-BetMCS | Insertion and replacement of Bet residues at amino acid 117 by insertion of a multiple cloning site | Fully susceptible towards feA3-mediated restriction in vitro |
| pCF7-Vif-4 | FFV-Vif-4 | Engineered Bet-Vif fusion protein | Partially susceptible towards feA3-mediated restriction in vitro |
| pCF7-Vif-39 | FFV-Vif-39 | Spontaneous frameshift | Fully susceptible towards feA3-mediated restriction in vitro |
| pCF7-Vif W/*1 | FFV-Vif W/*1b | Trp to Stop mutation (TGG to TGA), unlinked vif gene | Enhanced, compared to pCF7-Vif-4 |
| pCF7-Vif W/*2 | FFV-Vif W/*2 | Trp to Stop mutation (TGGG to TAGA), unlinked vif gene | Enhanced, compared to pCF7-Vif-4 |
| pCF7-Vif W/*1 M+ | – | Optimized upstream Met codon in pCF7-Vif W/*1 | Similar to pCF7-Vif W/*1 |
| pCF7-Vif W/*2 M+ | – | Optimized upstream Met codon in pCF7-Vif W/*2 | Similar to pCF7-Vif W/*2 |
| pCF7-Vif W/*1 M/T | – | Upstream Met codon mutated to Thr in pCF7-Vif W/*1 | Similar to pCF7-Vif W/*1 |
| pCF7-Vif W/*2 M/T | – | Upstream Met codon mutated to Thr in pCF7-Vif W/*2 | Similar to pCF7-Vif W/*2 |
aFFV-Vif variants collectively referred to as “FFV Vif chimeras”
bViral stocks used in domestic cat infection experiments