Table 2.

Primers used for cloning and PCR detection

Primer name	Sequence (5′–3′)
pCF7-Vif cloning
FFV-Vif #1	GCGGGCTAGCGCCGCGGTACACACCGTCAAAGCCATGAGCGAGGGGACTGGCAG
FFV-Vif #2	GTGCTCTCCAAAGACCGGTTATCACAGCTCGCCGCTCCACAGCAGATTCC
FFV-Vif #3	GGCGAGCTGTGATAACCGGTCTTTGGAGAGCACAAGCTGATG
FFV-Vif #4	CGCTCTGTTGCATGCCG
Mutagenesis of the upstream start codon
FFV 9233-F	GCGGTCCGGAACACCCAAGACGGATCCTACTCG
M/T-R	CGGCGCTAGCTCTAGTTAGCGTAGTCAAATCCCTCTCCCCAC
M+-R	CGGCGCTAGCTCTAGTTACCATAGTGAAATCCCTCTCCCCAC
PCR amplification of in vitro-selected FFV-Vif variants
FFV 9366-F	CCACTTCTGTTTGGACCTTACC
FFV-10288-R	CAGCTTGTGCTCTCCAAAGC
Nested FFV PCR
FFVgag-F1	CTACAGCCGCTATTGAAGGAG
FFVgag-R1	CCCTGCTGTTGAGGATTACC
FFVgag-F2	TTACAGATGGAAACTGGTCCTTAGT
FFVgag-R2	CATCAGAGTGTTGCTGTTGTTG
Real-time quantitative PCR
FFVgag -F	GGACGATCTCAACAAGGTCAACTAAA
FFVgag-R	TCCACGAGGAGGTTGCGA
FFVgag-TM	AGACCCCCTAGACAACAACAGCAACACT