Table 1.
Strains and plasmids used in this study
| Name | Relevant characteristics | Reference or source |
|---|---|---|
| Strain | ||
| Escherichia coli JM109 | recA1 endA1 gyrA96 thi hsdR17(r−K m+K) e14− (mcrA) supE44 relA1 Δ(lac-proAB)/F’ [traD36 proAB+ lacIq lacZΔM15] | TakaraBio |
| Corynebacterium glutamicum ATCC13032 | Type-strain of C. glutamicum | ATCC |
| C. glutamicum ATCC31831 | Wild type | ATCC |
| ΔaraR | Markerless araR-deletion mutant of strain ATCC31831 | This study |
| 31831/pCH | C. glutamicum ATCC31831 bearing pCH | This study |
| 31831/pCHpyk | C. glutamicum ATCC31831 bearing pCHpyk | This study |
| ΔaraR/pCH | ΔaraR bearing pCH | This study |
| ΔaraR/pCHpyk | ΔaraR bearing pCHpyk | This study |
| Plasmid | ||
| pCH | Kmr; E. coli–Corynebacterium sp. shuttle vector | [40] |
| pCHgltA | Kmr; pCH with PCR fragment containing C. glutamicum ATCC31831 gltA gene (NCgl0795) encoding citrate synthase | |
| pCHpfk | Kmr; pCH with PCR fragment containing C. glutamicum ATCC31831 pfk gene (NCgl1202) encoding phosphofructokinase | |
| pCHpyk | Kmr; pCH with PCR fragment containing C. glutamicum ATCC31831 pyk gene (NCgl2008) encoding pyruvate kinase | This study |
| pK19mobsacB | Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα) | ATCC |
| pK19mobsac-ΔaraR | Kanr, pK19mobsacB with the deletion construct for araR of C. glutamicum ATCC31831 | This study |