Table 2.
Oligonucleotides used in this study
| Name | Target gene | Sequence (5′-3′) | Cohesive endsa |
|---|---|---|---|
| Primer 1 | gltA | CTCTGGATCCGACTACTTCCGTAATCCGGA | BamHI |
| Primer 2 | gltA | CTCTCTGCAGCCGGTAGCTCAATCTGTGGC | PstI |
| Primer 3 | pyk | CTCTGGATCCATGGTAGTACCTGTGGCTTG | BamHI |
| Primer 4 | pyk | CTCTCTGCAGATGCTCTGCTCAAGAAGTGC | PstI |
| Primer 5 | pfk | CTCTGGATCCATAAGATGGTCAGAGACAGT | BamHI |
| Primer 6 | pfk | CTCTGAGCTCAGTCAAGCCTAGGTCACAGT | SacI |
| Primer 7 | pCH | GGATCCGATATCCTGCAGGAG | |
| Primer 8 | pCH | CTCGACCAACAGTTGCGCAGC | |
| Primer 9 | araR | CTCTCTGCAGGCGCTCAATGCTTGACAGCG | PstI |
| Primer 10 | araR | CTCTAAGCTTCCGACGGCATCTACACCGAT | HindIII |
| Primer 11 | araR | CTCTGAATTCTCGTGGAGGTTTCGCAGGAA | EcoRI |
| Primer 12 | araR | CTCTCTGCAGGCTGCCAATGACCAGATGGC | PstI |
a The restriction site overhangs used in the cloning procedure are underlined