Effect of prochelators on cell cycle in MDA-MB-231 cells. Cells were treated with the compounds (10 or 50 μM, 12 h), harvested, fixed, pelleted and then treated with RNAse and propidium iodide (0.5 mg/mL and 40 μg/mL, respectively, 30 min) prior to analysis by flow cytometry. Values are presented as mean ± SDM (n=3), * p < 0.05 and ** p < 0.01.