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Effect of serial tube transfer on cell media Aβ. Results in CM show the concentration of Aβ42, Aβ40, and Aβ38 decreased with consecutive transfer of sample to new storage tubes (A, C, E). Concentration of Aβ42 decreased proportionally more than Aβ40 (B) and Aβ38 (D) with each transfer, resulting in a decrease in the Aβ42:Aβ40 and Aβ42:Aβ38 ratios. (F) In turn, the rate of Aβ40 decrease with each transfer was greater than Aβ38. Abbreviations: CM, culture media; Aβ, amyloid β.
