Figure 2.

Cbln1 and Cbln2 single KOs and Cbln1/2 dKOs cause region-specific increases or decreases in synaptic proteins as assessed by quantitative immunoblotting. Representative blots of the indicated proteins (top) and summary graphs of their levels (bottom) measured by quantitative immunoblotting in lysates from cerebellum, motor cortex, caudate putamen, and thalamus. Lysates were obtained from littermate control, Cbln1, Cbln2, and Cbln1/2 dKO mice (age = 6 months; n = 3 mice). All data are shown as means ± SEM and were normalized first to the levels of β-actin as an internal loading control and then to the levels observed in the controls. Asterisks denote statistically significant differences between mutant and control samples as assessed by one-way ANOVA followed by Dunnet's multiple-comparisons correction. *adjusted p ≤ 0.05; **adjusted p ≤ 0.01.