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. 2018 Apr 9;8(10):2739–2751. doi: 10.7150/thno.21477

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Twist promotes the expression of ROR1. (A-B) Representative heatmaps from global comparative transcriptome analysis indicating genes that are up-regulated upon Twist overexpression. (C) The relative mRNA levels of the indicated genes were normalized to the GAPDH level in the T47D cells stably transfected with pLenti6.3/V5-vector or pLenti6.3/V5-Twist as determined by qRT-PCR. The results are expressed as the mean ± SD of three independent experiments. * P < 0.05 using Student's t-test. (D) The indicated proteins were analyzed by Western blotting in T47D cells stably expressing pLenti6.3/V5-vector or pLenti6.3/V5-Twist, as indicated. (E) T47D cells stably expressing pLenti6.3/V5-vector or pLenti6.3/V5-Twist were analyzed by immunofluorescence as indicated. Scale bars, 50μm.