Figure 2.
miR503HG inhibits HCC cell invasion and metastasis in vitro and in vivo. (A-B) Migration assays of 5×104 HCC cells or invasion assays of 1×105 HCC cells with miR503HG knockdown by using siRNA-1#. Representative images are shown on the left, and the average number of cells per field at the indicated time points is shown on the right. Data are the mean ± SD. **P < 0.01 and ***P < 0.001. Scale bar = 100 μm. (C-D) Migration and invasion assays of SMCC-7721 and Huh7 cells that stably overexpress of miR503HG or control vector. Data are the mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001. Scale bar = 100 μm. (E) Hematoxylin and eosin (H&E) staining of metastatic nodules in the lungs of nude mice 8 weeks after tail vein injection of Huh7 cells infected with lentivirus expressing miR503HG or the control. The numbers of metastatic nodules in the lungs of each mouse were counted and analyzed using Student's t-test. Data are presented as the mean ± SD (n = 9 mice per group). Scale bar = 200 μm (100x) or 50 μm (400x). (F) SMMC-7721 cells infected with lentivirus expressing miR503HG or the control vector were orthotopically injected into the livers of nude mice. 8 weeks later, the mice were sacrificed, and the livers were subjected to H&E staining. The numbers of liver metastatic nodules of each mouse were counted and analyzed using Students' t-test. Data are presented as the mean ± SD (n = 8 mice per group). Scale bar = 200 μm (100x) or 50 μm (400x).