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. 2018 Apr 15;8(10):2830–2845. doi: 10.7150/thno.23209

Figure 4.

Figure 4

Co-delivery of HPMC NPs, co-localization with intracellular MMP-3, and MMP sensitivity. (A) Intracellular uptake of dual-labelled HPMC NPs at 0 h, 1 h, 2 h and 4 h measured by flow cytometry after incubation at 37 °C at a FITC or RITC concentration of 800 ng/mL. Dual fluorophore-labelled HPMC NPs were prepared by conjugating FITC (green) to HA-PTX and RITC (red) to CN. (B) CLSM observation of intracellular dual-labelled HPMC NPs at 4 h post incubation at a FITC or RITC concentration of 800 ng/mL. (C) Co-localization of Rho-labelled HMPC NPs (red) and Alexa Fluor 488 (green)-labelled MMP-3 in 4T1 cells observed by CLSM. Cells were cultured with Rho-HPMC NPs at a Rho concentration of 800 ng/mL for 4 h at 37 °C. MMP sensitivity: (D) size change of Rho-MATT/CN complexes or Rho-HPMC NPs after culturing with pH 6.5 PBS containing MMP-3 and (E) Rho or (F) PTX release profiles in pH 6.5 PBS containing a 5 µg/mL MMP-3 at 37 °C. The data are presented as mean ± s.e.m. (n = 3, *P < 0.05).