Figure 3.

The enhancement of GC cell proliferation and cell cycle progression by lncRNA GAPLINC was dependent on MAPK1. GC cell line KATO III was transfected with control, pCDNA-GAPLINC, or pCDNA-GAPLINC + MAPK1 siRNA. (A) Cell proliferation was measured by CCK-8 assay at the indicated times in GC cell line KATO III. Results are represented as the mean ± SD. n = 5, ANOVA, *P < 0.05. (B) Cells were stained with propidium iodide (with RNase A). Cell cycle assay was measured using flow cytometry. Results are represented by three independent experiments. (C) The data of cell cycle were analyzed using Modifit software. Results are represented as the mean ± SD. n = 3, ANOVA, *P < 0.05. (D) KATO III cells were transfected with control, pCDNA-GAPLINC, or pCDNA-GAPLINC + MAPK1 siRNA. The cells (10⁵) were injected subcutaneously into the subcutis on the right flank of nude mice. Surgical resections of KATO III xenograft tumors on week 4 for animals and measurements of tumor volumes are shown. Results are represented as the mean ± SD. n = 5 per group, ANOVA, *P < 0.05.

Abbreviations: GC, gastric cancer; lncRNA, long noncoding RNA; CCK-8, cell counting kit-8.