Skip to main content
. Author manuscript; available in PMC: 2018 May 17.
Published in final edited form as: Mol Cell. 2018 Jan 27;69(3):412–425.e6. doi: 10.1016/j.molcel.2017.12.029

Figure 5. Rescue of Functional Defects on Cellular Models by RNASEH1 Overexpression.

Figure 5

(A and B) Proliferation of HEK293T cells stably expressing RNASEH1 with or without induced expression of SRSF2(P95H) (A, n = 3 biological replicates) or U2AF35(Q157P) (B, n = 4 biological replicates) upon Dox treatment for different days. Cells expressing empty vector (EV) served as a control.

(C and D) Quantitative analysis of γ-H2AX foci detected by immunocytochemistry in HEK293T cells expressing RNASEH1 before and after induced expression of SRSF2(P95H) (C) or U2AF35(Q157P) (D) by Dox treatment for 3 days. More than 100 cells per Dox treatment condition per cell type were analyzed, and p values were calculated by one-way ANOVA test.

(E and F) Suppression of Chk1 activation by overexpressed RNASEH1 in HEK293T cells upon induced expression of SRSF2(P95H) (E) or U2AF35 (Q157P) (F) by Dox for different days.

See also Figure S6A for RNASEH1 overexpression by western blotting analysis and Figures S6B–S6D for cell proliferation of additional mutant cells with or without RNASEH1 overexpression.