Table 1.
E. coli strains.
| E. coli strain | Genotypes and phenotypes |
|---|---|
| BW25113 GFP1 WT GFP (2 P, 5 SL) |
K12 strain, no O-antigen, core OS with 2 heptose layers and 3 glucose layers above Kdo layer, both heptose layers phosphorylated |
| JW3605-1 GFP1 ΔwaaP GFP (0 P, 5 SL) |
BW25113 derivative, core OS with 2 heptose layers (HepIII missing) and 3 glucose layers above Kdo layer, core OS dephosphorylated |
| JW3596-1 GFP1 ΔwaaC GFP (0 P, 0 SL) |
BW25113 derivative, core OS lacking all five sugar layers above Kdo layer, core OS dephosphorylated |
| BW25113 WT (2 P, 5 SL) |
K12 strain, no O-antigen, core OS with 2 heptose layers and 3 glucose layers above Kdo layer, both heptose layers phosphorylated |
| JW3602-1 ΔwaaI (2 P, 3 SL) |
BW25113 derivative, core OS with 2 heptose layers and 1 glucose layer above Kdo layer, both heptose layers phosphorylated |
| JW3600-1 ΔwaaY (1 P, 5 SL) |
BW25113 derivative, core OS with 2 heptose layers and 3 glucose layers above Kdo layer, phosphorylation at HepII missing |
| JW3592-1 ΔwaaF (1 P, 1 SL) |
BW25113 derivative, core OS with 1 phosphorylated heptose layer above Kdo layer |
1
These strains were transformed with a plasmid expressing GFP that is exported to the periplasm for two-color imaging experiments. See Methods for details of strain construction.