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. Author manuscript; available in PMC: 2019 Mar 6.
Published in final edited form as: Biochem J. 2018 Mar 6;475(5):873–886. doi: 10.1042/BCJ20170884

Figure 6. Levels of intracellular and secreted full-length FVIII mutants and their relative activities.

Figure 6

(A) and (B) Forty-eight hours after transfection into 293T cells with constructs of WT and the indicated mutants of full-length FVIII, cell extracts (A) and conditioned media (B) were collected. FVIII antigen levels measured by ELISA were plotted as percentages of WT FVIII (data are mean ± SEM, n = 3, *P < 0.05). (C) Forty-eight hours after transfection, FVIII activity levels in conditioned media were analyzed by a chromogenic assay. Average activities of mutants were plotted as percentages of WT FVIII activity (n = 3). Activities of all the mutants were significantly reduced compared with WT FVIII (P < 0.05). (D) Relative activity for a given mutant was calculated as the ratio of the activity level and the antigen level in conditioned media. Relative activities are shown as percentages of the WT FVIII relative activity. Relative activities of all mutants were significantly reduced compared with WT FVIII (P < 0.05).