Fig 1. Impaired expansion of popliteal lymph node post BCG injection in mice with H. polygyrus.
C57BL/6 mice (A, B) or Ly 5.1 mice (C) with chronic H. polygyrus were injected with 1x106 CFU of Bacille Calmette-Guérin (BCG) in the skin of the footpad. (A) The expansion of the foot draining popliteal LN was evaluated at different time points. (B) Correlation between the cellularity of the popliteal LN and the number of migratory (MHC-IIhi CD11c+) dendritic cells tracked by CFSE between 48 and 72 hours after BCG injection (C). Correlation between the number of mycobacteria specific congenic P25-TCRTg-ECFP T cells 6 days after BCG infection and the cellularity of the popliteal LN. In B and C, the numbers of cells were determined by FACS. Data in B is representative of two or more experiments and in C pooled from two replicate experiments. 1x105 P25-TCRTg-ECFP cells were injected i.v. the day before BCG infection. Parts of the data used to generate Fig 1B and 1C have also been use in a previous publication [2].