Fig 5. Nuceloprotein pSLA-I tetramer staining of bronchoalveolar lavage samples from Babraham pigs vaccinated with H1N1 S-FLU.

Babraham pigs were either left unvaccinated (1 and 2) or received H1N1 S-FLU via aerosol administration (6, 7, 8). H1N1 S-FLU vaccinated animals received a boost at day 28 with the same vaccine. Animals were culled and bronchoalveolar lavage harvested at day 57. Nucleoprotein and irrelevant peptide SLA-I tetramer staining was performed on thawed bronchoalveolar lavage samples and the percentage of tetramer⁺ cells of CD8β⁺ cells displayed in red. The sequences of the nucleoprotein peptides are shown. Irrelevant tetramers: SLA-1*14:02-AFAAAAAAL and SLA-2*11:04-GAGGGGGGI. Gating strategy: lymphocytes, single cells, viability (Vivid^neg) CD3⁺ CD14^neg then CD8β⁺ CD4⁺ and displayed as CD8β versus tetramer (S1 Fig).