Fig 3. A representative sample of nucleotide sequences of the eye color mutants and wild type eye color insects from CRISPR injection experiments.

Sequences IM 1 through IM 24 and IW 1 through IW 24 are from insects with mutant and wild type eye color phenotype, respectively, from CRISPR/Cas9 injections. Partial nucleotide sequences of exon 6 of the TO gene from the reference sequence (Ref) and uninjected wild type (WT) are also shown. Nucleotides identical to the exon 6 of the reference sequence (accession: MF598173) are indicated by a dot (.) and alignment gaps are shown by a hyphen (-). Nucleotides that differ from the reference are indicated on each sequence. Target (protospacer) sequences for which crRNA were designed are underlined with dotted lines and protospacer adjacent motifs (PAM) are underlined with a solid line.