Table 1. Average number pf eggs injected, average hatch count, corrected hatch count, percentage of adults emerged from larvae, and the percentage of mutants from control, mock injected (with injection buffer), and 1, 2, and 4 μM ribonucleoprotein complexes targeting exon 6 of the tryptophan 2,3-dioxygenase gene in Helicoverpa zea.
Total # eggs (Avg.) | Avg. Hatch count±SD | Corrected Avg. Hatch count | % adults emerged±SD | % mutants±SD‡ | |
---|---|---|---|---|---|
Uninjected Control | 448 (112.0) | 42.3±2.6 | 100.0 | 87.1±3.5 | 0.0 |
Mock Injected | 560 (116.0) | 32.3±5.2 | 80.5 | 87.6±4.3 | 0.0 |
1.0 μM injected | 488 (122.0) | 32.2±3.3 | 87.2 | 79.1±7.5 | 48.1±15.1a |
2.0 μM Injected | 570 (142.5) | 30.0±5.0 | 74.7 | 83.3±5.9 | 88.8±3.2b |
4.0 μM Injected | 541 (135.3) | 35.9±5.0 | 78.6 | 77.7±15.2 | 93.5±6.3b |
‡ Percentages of mutants from CRISPR/Cas9 injections that were significantly different are indicated by different letters.