Fig 4. Effect of AZA on iron sensing gene expression in HepG2 cells.

Cells were grown in the presence or absence of the global demethylating agent AZA (5 μM, 72 h). Expression of (a) HAMP, (b) TFR2 alpha, transcript, (c) TFR2 beta transcript, (d) HFE, (e) BMP6 and (f) HFE2 (HJV) were measured by RT-PCR. Data have been normalised to the control group and are presented as mean ± SEM of 7–8 observations in each group. Data were analysed using Mann-Whitney tests. * P < 0.005; ** P < 0.03.