Table 1. PCR primers sequences.

Primer	Forward sequence	Reverse Sequence
B2M RT	CCA CTG AAA AAG ATG AGT ATG CCT	CCA ATC CAA ATG CGG CAT CTT CA
HAMP RT	CTG CAA CCC CAG GAC AGA G	GGA ATA AAT AAG GAA GGG AGG GG
TFR2-α RT	GTC AGT GAG GAT GTC AA	CCA CAC GTG GTC CAG CTT CT
TFR2-β RT	CCA GAA AAG TCC CCA CCT C	TGC TCT CCG ACC TTC CC
HFE RT	AGA ACA GGG CCT ACC TGG AG	TGT GTC ACC TTC ACC AAA GG
HFE2 (HJV) RT	GGA GCT TGG CCT CTA CTG GA	ATG GTG AGC TTC CGG GTG
BMP6 RT	CCG TGT AGT ATG GGC CTC AGA	TCA CAA CCC ACA GAT TGC TAG T
TFR2-α BIS	GGG GGT TGA GGG ATT AGA GAA	CCA AAA CTA TAC CCC CAC CCT TAA AA
HAMP Met	TTT TGT TTT CGT TTA TTT TTT TCG T	AAA CTC AAT ACC ATC GTA CCG TC
HAMP Unmet	TTT TGT TTT TGT TTA TTT TTT TTG T	AAA AAC TCA ATA CCA TCA TAC CAT

RT = qRT-PCR primer; BIS = primers for bisulphite-converted DNA; Met = specific primers for methylated bisulphite-converted DNA; Unmet = specific primers for unmethylated bisulphite-converted DNA.