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. 2018 May 17;13(5):e0197184. doi: 10.1371/journal.pone.0197184

Fig 3. Calibration curve of qPCR singleplex and duplex assays with tenfold serially diluted CLas-infected ACP DNA (10 ng to 0.0001 ng) using 16S (unbroken line) and RNR (broken line) primers.

Fig 3

(a) The singleplex qPCR assay efficiency for 16S and RNR DNA standard curve is 100.42% and 91.33%, respectively. (b) The duplex qPCR assay is 100.87% and 97.56%, respectively.