Fig 1. ß-catenin activity in mouse AnxA6-/- and WT articular chondrocytes.

A: ß-catenin activity was determined by luciferase activity from the TOPFlash (TOP) or FOPFlash (FOP) luciferase reporter in vehicle-treated, Wnt3a-treated, and BAPTA-2AM/Wnt3a-treated WT and AnxA6-/- mouse articular chondrocytes. B: ß-catenin activity was determined by luciferase activity from the TOPFlash (TOP) or FOPflash (FOP) luciferase reporter in vehicle-treated, and Wnt3a-treated WT or AnxA6-/- mouse articular chondrocytes that were transfected with empty pcDNA expression vector (pcDNA) or pcDNA expression vector containing full-length AnxA6 cDNA (AnxA6). Mouse articular chondrocytes after transfection in A and B were serum starved followed by treatment with Wnt3a or the intracellular Ca²⁺ chelator, BAPTA-2AM followed by Wnt3a. Cell extracts were then analyzed for luciferase activity 48 h post-transfection. Transfection efficiency was monitored by co-transfection with Renilla luciferase vector, which provides constitutive expression of the Renilla luciferase reporter. Values were normalized to Renilla luciferase activity. Data are expressed relative to the normalized luciferase activity levels from the TOPFlash reporter of vehicle-treated WT cells, which was set as 1. Data (n = 4) are expressed as mean ± SD. (*p < 0.05; **p < 0.01; ***p < 0.001).