Table 6. Fusion detection in Archer FusionPlex Sarcoma Assay libraries with total number of unique reads and down-sampled number of reads for each sarcoma sample separately.
| Sample | Fusion | AllPrep Full/Down-sampled |
FormaPure Full/Down-sampled |
RNeasy Full/Down-sampled |
truXTRAC R/D Full/Down-sampled |
||||
|---|---|---|---|---|---|---|---|---|---|
| SARC3 | SS18-SSX2 | No 62k (4.5) |
No 62k (4.5) |
Yes 141k (9.0) |
Yes 127k (9.5) |
No 70k* (4.7) |
No 70k (4.7) |
Yes 187k (10.1) |
Yes 128k (9.9) |
| SARC4 | EWSR1-NR4A3 | Yes 190k (10.2) |
Yes 153k (11.0) |
Yes 184k (10.8) |
Yes 152k (11.4) |
Yes 109k* (7.9) |
Yes 109k (7.9) |
Yes 175k (8.7) |
Yes 132k (9.5) |
| SARC5 | EWSR1-FLI1 | No 15.4k (1.1) |
No 13.7k (1.2) |
No 6.5k* (0.6) |
No 6.5k (0.6) |
No 5.8k (1.4) |
No 5.8k (1.4) |
No 40k (0.6) |
No 15.6k (1.3) |
Yes–fusion detected, No–fusion not detected. The total number and percentage (%) of unique fragments identified in libraries are given. The down-sampling was done according to the sample with lowest total number of reads (> 1.5 million reads) (labelled with *). See S1 Table for more detailed information.