Fig 3. Slow exchange and amide CSPs from CBAP-acylation of BlaR^S.

(A) Residues demonstrating slow chemical exchange (orange spheres) and significant CSPs (blue spheres) are mapped onto PDB 3Q7Z. Spheres indicate residues assigned in both apo and CBAP-acylated BlaR^S. Green sticks represent the bound β-lactam CBAP. (B) Pronounced ¹⁵N^H-¹H exchange squares of V532 and Y536 of U-[¹⁵N] 80% deuterated CBAP-acylated BlaR^S. Spectra were recorded at 18.8 T and T(nom) = 293.8 K; ¹⁵N TROSY (Blue) versus EXSY-R₁ TROSY (black) using a 400 ms exchange relaxation delay.