Fig 6. Ank1 and Ank6 translocate into the nucleus and prevent p65 nuclear accumulation.

(A) HeLa cells were transfected to express Flag-tagged BAP, Ank1, Ank6, or IκBα SR. At 16 h, the cells were exposed to TNFα or vehicle control for 30 min, after which they were fixed, screened with antibodies specific for the Flag epitope and p65, and examined by confocal microscopy. (B) The mean percentage + SD of transfected cells exhibiting p65 in the nucleus was determined. Triplicate samples of 100 cells each were counted per time point. Statistically significant (****P < 0.0001) values indicated. Results are representative of three independent experiments.