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. 2018 May 7;14(5):e1007023. doi: 10.1371/journal.ppat.1007023

Fig 7. Ank1 and Ank6 translocate into the nucleus.

Fig 7

(A) HeLa cells were transfected to express Flag-tagged BAP, Ank1, Ank6, or IκBα SR. At 16 h, they were treated with TNFα or vehicle control (Ctrl) for 30 min. Western blotted nuclear or cytosolic fractions were screened with antibodies against p65, lamin B1, GAPDH, or the Flag epitope. (B) Mean normalized ratios + SD of p65:lamin B1 densitomery signals were calculated from three separate experiments performed in (A). Statistically significant values (*P < 0.05; ***P < 0.001) are indicated. Data presented are representative of three separate experiments.