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. 2018 May 7;14(5):e1007023. doi: 10.1371/journal.ppat.1007023

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© 2018 Evans et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 9 — Whole cell lysates of HeLa cells expressing Flag-tagged BAP, Ank1, Ank6, or Ank7 were incubated with Flag antibody-conjugated agarose beads to immunoprecipitate Flag-tagged proteins and their interacting proteins. Resulting Western blots were probed with antibodies specific for importin β1, exportin 1, and p65. Expression of each Flag-tagged protein of interest was confirmed by subjecting input lysate to Western blotting using Flag antibody. Data presented are representative of three independent experiments.