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. 2018 May 7;14(5):e1007023. doi: 10.1371/journal.ppat.1007023

Fig 10. Ank1 and Ank6 require importin β1 activity to optimally translocate into the nucleus.

Fig 10

(A) HeLa cells were transfected to express Flag-tagged Ank1, Ank6, or Ank9. At 16 h, the cells were treated with importazole or vehicle for 3 h. Following lysis and nuclear fractionation, Western blotted nuclear or cytosolic fractions were screened with antibodies against the Flag epitope, lamin A/C, and GAPDH. (B) Mean ratios + SD of Flag:lamin A densitometric signals from three separate experiments were normalized to Flag-Ank9 to account for the low level background nuclear accumulation of the ectopically expressed Anks. Statistically significant (*P < 0.05) values are indicated. n.s., not significant. Data presented are representative of three independent experiments.