Fig 16. Identification of the Ank1 and Ank6 domains that are essential for optimal translocation into the nucleus.

HeLa cells were transfected to express Flag-tagged versions of Ank1, Ank6, or the indicated deletion mutant thereof. At 16 h, the cells were fixed, screened with Flag tag antibody, stained with DAPI, and examined by confocal microscopy. (A and B) Representative fluorescence images of cells viewed for Flag-tagged Ank1 (A) or Ank6 proteins (B) with and without DAPI. (C) The mean percentage + SD of transfected cells exhibiting Flag signal accumulation around the periphery of nuclei was determined. Triplicate samples of 100 cells each were counted per condition. Data presented are indicative of three experiments with similar results. Statistically significant (*P<0.05; **P<0.01; ***P<0.001; ****P<0.0001) values are indicated. n.s., not significant. Indicators of statistical significance relative to values obtained for Flag-Ank1, Flag-Ank1△ISR, Flag-Ank6, and Flag-Ank6△ISR are colored red, black, blue, and black, respectively.