Fig 4. Cd2ap-deficiency enhances T_FH differentiation in a cell-intrinsic manner.

(A) Schematic of experimental strategy for the generation of mixed BM chimeras and subsequent analysis of anti-LCMV responses. (B) Contribution of CD45.2 Cd2ap^F/F or Cd4-cre⁺ Cd2ap^F/F cells to B cells, activated CD4 T cells and T_FH cells in the mixed BM chimeras 22 days after LCMV-c13 infection. Representative Data from 2 independent experiments (n = 4 of each genotype) are shown with means and standard deviations. Statistical difference was tested by Student’s t-test. (C) A volcano plot showing differentially expressed genes between Cd2ap-deficient (KO, n = 2) and -sufficient (WT, n = 2) T_FH cells harvested from mixed BM chimeras 22 days after infection. Genes that were differentially expressed by >2-fold are shown.